Chapter Outline: Genomic Features of Dioxin-Like Compound Degraders (Shintani and Kimbara)

Outline of Manual of Environmental Microbiology, 4th ed., book chapter

Proposed title: Genomic Features of Dioxin-Like Compound Degraders   Chapter no.:_______

Author(s):      Masaki Shintani (PhD) and Kazuhide Kimbara (PhD)

                        Department of Applied Chemistry and Biochemical Engineering
                        Graduate School of Engineering
                        Shizuoka University
                        3-5-1 Johoku, Naka-ku, Hamamatsu 432-8561, Shizuoka, Japan
                        Phone:             +81-53-478-1170
                        Fax:                +81-53-478-1170
                        E-mail:            tkkimba@ipc.shizuoka.ac.jp

Notes:

·       Agrochemicals of POPs should be described in other chapter.

            ·       Dioxin-like compounds include PCB, dioxin, and dibenzofuran.
            ·       Relationship between genomic features and physiological effects will be discussed.

Proposed topics

1)  Recent progress on genome analysis of dioxin-like compounds-degraders

2)  Multiple copies of dioxygenase in Rhodococcus jostii RHA1 and other degraders
3)  Genomic islands and other mobile genetic elements which carry degradative genes
4)  How microorganisms have acquired degradation ability
5)  Transcriptome and proteome analyses on degradation of dioxin-like compounds
6)  Physiologic features of degradation
7)  Effects on dioxin-like compounds and their metabolites on microbial population in environment
8)  Genomic and physiologic analyses for successful bioremediation
9)  New frontier: single-cell analysis on degraders in environments which include unculturable microorganisms

Chapter Highlights

The following concepts will be conveyed in this chapter:

1. Genome analysis of dioxin-like compounds-degraders

2. Mobile genetic elements for distributing degradation genes
3. Effects on dioxin-like compounds and their metabolites on microorganisms
4. Genomic and physiologic analyses for successful bioremediation

5. Future aspects of single-cell analysis on biodegradation