Proposed title: Microbial life in
extreme low-biomass environments – a genetic approach
Chapter no.: _________
Author(s): Venkateswaran,
K., M.T. La Duc, P. Vaishampayan, and J.A. Spry
Jet
Propulsion Lab, California Institute of Technology
M/S:
89-2; Biotechnology and Planetary Protection
4800,
Oakgrove Dr., Pasadena, CA 91109
Proposed topics
1.
ATP as a biomarker of
viable microorganisms in clean-room facilities.
2. Evaluation of Procedures for the
Collection, Processing, and Analysis of Biomolecules from Low-Biomass Surfaces.
3.
Comparative analysis
of methods for the purification of DNA from low-biomass samples based on total
yield and conserved microbial diversity.
4. Differential recovery of phylogenetically
disparate microbes from spacecraft-qualified metal surfaces.
B. Cultivable and problematic microbes of spacecraft and associated surfaces
7.
Recurrent isolation of
extremotolerant bacteria from the clean room where Phoenix spacecraft
components were assembled.
8.
Extreme spore UV
resistance of Bacillus pumilus
isolates obtained from an ultraclean spacecraft assembly facility.
9.
Recurrent isolation of
hydrogen peroxide-resistant spores of Bacillus
pumilus from a spacecraft assembly facility.
12. Effect of Shadowing on Survival of Bacteria
under Conditions Simulating the Martian Atmosphere and UV Radiation.
15.
Molecular microbial
community structure of the Regenerative Enclosed Life Support Module Simulator
air system.
16.
Impact of assembly,
testing, and launch operations on the airborne bacterial diversity within a
spacecraft assembly facility clean-room.
19.
Diversity of anaerobic
microbes in spacecraft assembly clean rooms.
21.
Comprehensive Census
of Bacteria in Clean Rooms by Using DNA Microarray and Cloning Methods.
22.
Comparison of
Innovative Molecular Approaches and Standard Spore Assays for Assessment of
Surface Cleanliness.
23.
High-density 16S
microarray and clone library-based microbial community composition of the
Phoenix spacecraft assembly clean room.
24.
Pyrosequencing-derived
bacterial, archaeal, and fungal diversity of spacecraft hardware destined for
Mars.
Chapter Highlights
The
following concepts will be conveyed in this chapter:
I.
Effectiveness of sample collection
methods and efficacy of sample processing in measuring molecular microbial
community of low biomass surfaces
II.
Are molecular methods comprehensive
enough to measure microbial diversity?
III.
Are clean surfaces selectively
enriching subset of microbial population?
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