Tuesday, March 26, 2013

Chapter outline: Iron and Microbial Life—from the Beginning to the Present Day


Proposed title: Iron and Microbial Life—from the Beginning to the Present Day

 
Chapter no.:                 _____________

 
Author:                            TS Magnuson

 
1.     Introduction

1.1.  An earth history of microbial life with metals

1.2.  Overall biogeochemical significance

1.3.  Importance of Fe transformation on early and present earth

1.4.  Evolution of mechanisms of microbial iron respiration

1.5.  Link to present day processes

2.     The present state of knowledge

2.1.1.     Geobacter and Shewanella

2.2.  Other microbial systems

2.2.1.     Desulfovibrio, thermophilic, extremophilic microbes studied thus far

2.3.  Methods for cultivation and study of Fe transforming microbes

2.3.1.     Use of proper substrates-solid vs. soluble Fe

2.3.2.     Biofilm physiology studies

2.4.  Genomics of Fe-transformation

2.4.1.     Methods used in genomic and proteomic studies

2.4.2.     Common genome features among Fe transforming microbes

2.5.  Biochemistry and physiology of Fe transformation

2.5.1.     Methods applied in the study of biochemistry/physiology

2.5.2.     The biofilm matrix—a redox active interface between cells and the mineral substrate

2.5.3.     Redox proteins involved in Fe transformation

2.5.4.     Other potential mechanisms

2.5.4.1.          Shuttles and redox partner bacteria

2.5.5.     Common themes among Fe transforming bacteria

2.6.  Areas of controversy

2.6.1.     Nanowires, membrane vesicles, and the biofilm matrix

2.6.2.     Gram positive and archaeal systems

3.     The happy consequences of microbial Fe transformation

3.1.  Transformation and bioremediation of toxic metals

3.2.  Cr and U transformation mechanisms

3.3.  Mechanisms and relatedness to Fe transformation activity

4.     The great beyond—Future directions for research

4.1.  Exploration of extremophilic microbial Fe transformation systems

4.2.  Bioremediation

4.3.  A common theme for microbial Fe transformation?

5.     Acknowledgments

6.     References

 

 

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